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1引言蛋白质测定在临床医学和生命科学研究中有重要意义，这方面的研究引起人们的广泛兴趣，最常用的方法是用染料结合法，它们在酸性介质与蛋白质作用形成复合物，而后进行测定，荧光法测定蛋白质文献报道较少。在pH2．53的介质中，我们研究曙红Y与牛血清蛋白作用光谱，曙红Y
1 Introduction Protein determination is of great importance in clinical medicine and life science research. The research in this area has attracted widespread interest. The most commonly used method is the dye binding method, which forms a complex in the action of acidic medium and protein and then measures There are few reports on the determination of protein by fluorescence method. In pH2.53 medium, we studied the interaction of Eosin Y with bovine serum albumin, Eosin Y