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目的:观察局灶性脑缺血再灌注后Nogo-A mRNA和蛋白表达的动态变化,以及三七三醇皂苷(PTS)对其影响。方法:将健康雄性SD大鼠随机分为假手术组、模型组和药物干预组(PTS组),采用Longa线栓法制备大脑中动脉阻塞与再灌注大鼠短暂局灶性脑缺血模型。药物干预后,采用RT-PCR结合免疫组织化学技术检测大鼠缺血再灌注后不同时期(3 d,7 d)脑组织Nogo-A mRNA和蛋白的表达。结果:模型组Nogo-A mRNA和蛋白表达在缺血再灌注损伤后3 d时下降,7 d时上升。PTS组在3 d时大脑皮层和海马Nogo-A表达比模型组低,但差异无显著性意义(P>0.05);7 d时Nogo-A表达明显低于模型组,差异有显著性意义(P<0.01)。结论:PTS可使缺血再灌注大鼠的Nogo-A表达下降,这可能是其发挥脑保护作用的机制之一。
Objective: To observe the dynamic changes of Nogo-A mRNA and protein expression after focal cerebral ischemia-reperfusion and the effect of PTS. Methods: Healthy male Sprague-Dawley rats were randomly divided into sham operation group, model group and drug intervention group (PTS group). The model of transient focal cerebral ischemia in middle cerebral artery occlusion and reperfusion rats was established by Longa suture method. After drug intervention, the expression of Nogo-A mRNA and protein were detected by RT-PCR and immunohistochemistry in different time periods (3 d, 7 d) after ischemia-reperfusion in rats. Results: The expression of Nogo-A mRNA and protein in the model group decreased 3 days after ischemia-reperfusion injury and increased on the 7th day. The expression of Nogo-A in cerebral cortex and hippocampus in PTS group was lower than that in model group at 3 d (P> 0.05), while the expression of Nogo-A at 7 d was significantly lower than that in model group P <0.01). CONCLUSION: PTS can decrease the expression of Nogo-A in rats following ischemia-reperfusion, which may be one of the mechanisms of its protective effects.