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动物实验肺结核空洞形成的目的,是为了研究肺结核空洞治疗等问题。我们从1960年9月起,先后利用了家兔和狗进行实验,发现用狗作实验肺结核空洞所获得的结果较用兔优越。现将我们初步研究的方法和结果介绍如下。实验方法及步骤我们采用的方法是根据日本山村和北京结核病研究所吴爱娣等家兔实验方法加以改良的。 (1)菌液的制备:本实验所用的肺内感染原,是以活的牛型结核干菌、胆固醇、无水羊毛脂、旧结素和液体石蜡,按下列剂量配成力混悬液。活牛型结核干菌 200毫克胆固醇 100毫克无水羊毛脂 1毫升液体石蜡 4毫升旧结核菌素原液 5毫升配治时先将称好的活牛型结核干菌放置经过高压
Animal experiments The purpose of the formation of tuberculosis cavity is to study the problem of tuberculosis treatment. Since September 1960, we have used rabbits and dogs to experiment and found that the results obtained by using dogs as experimental tuberculosis voids are superior to rabbits. The method and result of our preliminary study are introduced below. Experimental methods and procedures We used the method is based on Japanese mountain village and Beijing Institute of Tuberculosis Wu Aidi and other experimental methods to be improved rabbit. (1) Bacterial liquid preparation: In this experiment, the original lung infection was based on live bovine tuberculosis dry bacteria, cholesterol, anhydrous lanolin, nodules and liquid paraffin, according to the following dose dosing force suspension . Live cattle TB dry 200 mg Cholesterol 100 mg anhydrous lanolin 1 ml liquid paraffin 4 ml old tuberculin solution 5 ml When first weighed live cattle TB dry place after high pressure