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目的:研究双香豆素(Dic)对肿瘤细胞的光敏反应及二乙基二硫代氨基甲酸钠的增敏作用.结果:Dic 40,80,120 μmol·L~(-1)光敏反应使腹水型肝癌细胞(Hep A)死亡率增加(50％—70.4％),照光组始终高于避光组30％左右.照光组细胞DNA合成抑制率(81％—93％)明显高于避光组(19％—53％),且有浓度依赖性.避光组Dic对细胞也有轻微的损伤.结论:Dic光敏反应对Hep A细胞有较强抗肿瘤活性,DDC能增强Dic对细胞的光敏杀伤作用.Dic—DDC照光组细胞死亡率及DNA合成的抑制率比单独的Dic照光组明显增高.Dic光敏反应与~1O_2和OH无关,而与H_2O_2与O_2密切相关.
Objective: To study the photosensitivity of dicoumarin (Dic) on tumor cells and sensitization of sodium diethyldithiocarbamate. Results: Dic 40, 80, 120 μmol·L -1 photosensitivity to ascites liver cancer The death rate of Hep A cells increased (50%-70.4%), and the photosensitized group was always higher than 30% of the light-protected group. The inhibition rate of DNA synthesis in cells of the phototherapy group (81%-93%) was significantly higher than that of the dark group (19). %-53%), and there is a concentration dependence. Dic light group also has slight damage to cells. Conclusion: Dic photosensitive reaction has strong anti-tumor activity on Hep A cells, DDC can enhance the photosensitive killing effect of Dic on cells. The inhibition rate of cell death and DNA synthesis in the Dic-DDC group was significantly higher than that in the Dic group alone. Dic photosensitivity was not associated with ~1O_2 and OH but closely related to H_2O_2 and O_2.