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采用Fenton体系法、邻苯三酚自氧化法、DPPH法和ORAC法评价纳米硒-谷氨酸、纳米硒-甘氨酸和纳米硒-丙氨酸溶胶的抗氧化能力。结果表明:3种纳米硒-氨基酸溶胶清除OH-.能力在一定范围内随溶胶浓度的增加而增加,然后趋于平缓;溶胶清除O2-.能力与溶胶浓度以及其中纳米硒(nanoSe0)的量均呈现正相关,其溶胶的IC50值分别为(57.5±7.5)μmol/L(nanoSe0-Glu)、(6.78±0.23)μmol/L(nanoSe0-Gly)、(32.3±6.9)μmol/L(nanoSe0-Ala);3种溶胶都具有较强的清除DPPH.能力,其IC50值分别为(15.5±0.27)μmol/L(nanoSe0-Glu)、(15.3±0.89)μmol/L(nanoSe0-Gly)、(15.2±0.60)μmol/L(nanoSe0-Ala)。ORAC检验结果显示,3种纳米硒-氨基酸溶胶的荧光衰退曲线的延缓面积(AUC)与其浓度呈良好的正相关性,并且nanoSe0-Ala溶胶的总抗氧化能力强于nanoSe0-Glu溶胶和nanoSe0-Gly溶胶。研究结果表明纳米硒-氨基酸溶胶可以有效的清除O2-.、OH-.和DPPH.且总抗氧化活性较强,作为一种有效的抗氧化剂有望应用于食品防腐和食品保健等方面。
The antioxidant activity of nano-selenium-glutamic acid, nano-seleno-glycine and nano-selenium-alanine sol were evaluated by Fenton system, pyrogallol autoxidation method, DPPH method and ORAC method. The results showed that the ability of three kinds of nano-selenium-amino acid acid scavenging OH- increased with the increase of sol concentration within a certain range, and then tended to be gentle. The solute O2-, sol-gel concentration and the content of nanoSe0 (57.5 ± 7.5) μmol / L (nanoSe0-Glu), (6.78 ± 0.23) μmol / L (nanoSe0-Gly), (32.3 ± 6.9) μmol / L -Ala). The IC50 values of the three sols were (15.5 ± 0.27) μmol / L (nanoSe0-Glu), (15.3 ± 0.89) μmol / L (nanoSe0-Gly) (15.2 ± 0.60) μmol / L (nanoSe0-Ala). The results of ORAC showed that there was a positive correlation between the deferred area (AUC) and the total antioxidant capacity of nanoSe0-Ala sol and nanoSe0- Gly sol. The results showed that nano-selenium-amino acid sol could effectively remove O2-, OH-, and DPPH, and the total antioxidant activity is strong. As an effective antioxidant, it is expected to be applied to food preservation and food hygiene.