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傅里叶变换红外光谱结合多元统计方法应用于微生物的快速鉴定及分类是近几年发展起来的一门新型技术。文章用傅里叶变换红外光谱研究了镰刀菌属五种6株镰刀菌。在获得分辨率高、重现性好的镰刀菌全细胞组分的红外谱图基础上,分析了主要吸收峰的归属,确定了镰刀菌红外谱图3 000~2 800 cm-1,1 800~1 600 cm-1与指纹区3个分析灵敏区。根据分析灵敏区的差异性区分了不同种的镰刀菌,并在此基础上进行了系统聚类分析并对镰刀菌种间进化亲缘关系进行了比较。结果表明,可根据光谱峰位、峰值及吸收强度比鉴别镰刀菌,而系统聚类分析方法能更科学合理地鉴别镰刀菌及呈现种间相似性程度,但不适合反映镰刀菌遗传亲缘关系。傅里叶变换红外光谱技术所具有快速、精确,易操作等优点是必将成为微生物研究领域的一个重要工具。
The application of Fourier transform infrared spectroscopy combined with multivariate statistical methods in the rapid identification and classification of microorganisms is a new technology developed in recent years. In this paper, five Fusarium species of Fusarium were studied by Fourier transform infrared spectroscopy. Based on the infrared spectra of Fusarium solani with high resolution and good reproducibility, the main absorption peaks were analyzed. The Fusarium infrared spectrum was determined from 3000 to 2800 cm -1,1 800 ~ 1 600 cm-1 and fingerprint zone 3 sensitive area. According to the difference of sensitive zone, different species of Fusarium were distinguished, and based on this, the phylogenetic analysis was carried out and the phylogenetic relationships among Fusarium species were compared. The results showed that Fusarium species could be identified according to the ratio of peak position, peak value and absorption intensity. However, the cluster analysis method could identify Fusarium species more scientifically and reasonably, but not to reflect the genetic relationship of Fusarium species. Fourier transform infrared spectroscopy has the advantages of fast, accurate, easy to operate, etc. It is bound to become an important tool in the field of microbiology.