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目的:探讨振动运动和白藜芦醇对2型糖尿病大鼠肝脏损伤的保护作用及其机制。方法:采用高脂饮食和链脲佐菌素结合的方法建立大鼠2型糖尿病模型。实验分为:1正常组;2模型组;3振动运动组;4白藜芦醇组20 mg/kg组;5白藜芦醇40mg/kg组;6运动+白藜芦醇20 mg/kg组;7运动+白藜芦醇40 mg/kg组。末次给药或振动运动后血糖仪测大鼠空腹血糖,ELESA法测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平;HE染色观察肝组织形态学变化;电泳法测定肝组织NF-κBp65表达。结果:与正常组相比,模型组空腹血糖显著增加,血清ALT、AST、TNF-α、IL-6水平增加,肝组织核蛋白NF-κBp65表达增加。振动运动和白藜芦醇(20、40 mg/kg)都可改善上述病理变化,而振动运动联合白藜芦醇(20、40mg/kg)治疗对上述肝功能的改善以及炎症因子和NF-κBp65表达的抑制作用更为明显。结论:振动运动联合白藜芦醇对糖尿病肝损伤具有一定的保护作用,其作用机制与抑制NF-κB通路和炎症反应有关。
Objective: To investigate the protective effect of vibrational exercise and resveratrol on liver injury in type 2 diabetic rats and its mechanism. METHODS: A rat model of type 2 diabetes was established using a combination of a high fat diet and streptozotocin. The experiments were divided into: 1 normal group; 2 model group; 3 vibration exercise group; 4 resveratrol group 20 mg/kg group; 5 resveratrol 40 mg/kg group; 6 exercise + resveratrol 20 mg/kg Group; 7 Exercise + Resveratrol 40 mg/kg group. The fasting blood glucose was measured by glucose meter after the last administration or vibration exercise. Serum ALT, AST, TNF-α, IL-6 were measured by ELESA method. -6) level; HE staining was used to observe the morphological changes of liver tissue; electrophoresis was used to determine the expression of NF-κBp65 in liver tissue. Results: Compared with the normal group, the fasting blood glucose in the model group increased significantly, serum ALT, AST, TNF-α, IL-6 levels increased, and the expression of NF-κB p65 in liver tissue increased. Vibrational exercise and resveratrol (20, 40 mg/kg) both improved the above pathological changes, while vibrational exercise combined with resveratrol (20, 40 mg/kg) treatment improved the above liver function as well as inflammatory factors and NF- The inhibition of κBp65 expression was more pronounced. Conclusion: Vibratory movement combined with resveratrol has a certain protective effect on diabetic liver injury, and its mechanism of action is related to inhibition of NF-κB pathway and inflammatory reaction.