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在时间分辨荧光免疫分析(TR-FIA)体系中,通常以镧系元素Eu~(3+)标记抗体(抗原)的螯合物作标记物,当特异性抗原(或抗体)专一地形成抗体一抗原(或抗原-抗体)复合物,其荧光强度很弱 经解离增强溶液作用,Eu~(3+)可解离出来,形成一种新复合物(螯合物),它在紫外光的激发下,发出高强度的荧光,从而对被测物定量。作者自制增强溶液并和LKB增强溶液进行了荧光和紫外光谱分析和比较。 1 材料和方法 1.1 材料 三辛基磷化氧氧化物(TOPO,Merck),
In the time-resolved fluorescence immunoassay (TR-FIA) system, the chelate of the lanthanide Eu 3+ labeled antibody (antigen) is usually used as a marker. When specific antigens (or antibodies) are specifically formed Antibody-antigen (or antigen-antibody) complex, its fluorescence intensity is very weak by the dissociation enhancement solution, Eu ~ (3+) can be dissociated to form a new complex (chelate) Light excitation, issued a high-intensity fluorescence, thus measuring the amount of material. Authors made enhancement solutions and compared LGF enhancement solutions with fluorescence and UV spectroscopy. 1 Materials and methods 1.1 Materials Trioctyl phosphine oxide (TOPO, Merck),