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目的研究蒸制轮叶党参(steamed Codonopsis lanceolata,SCL)对乙醇暴露致急性肝损伤小鼠的保护作用。方法雄性ICR小鼠随机分为6组,即空白对照组、模型组、水飞蓟素组及SCL低、中、高剂量组。除空白对照组外,其余各组建立急性酒精性肝损伤小鼠模型。各组给予相应干预措施。采用比色法测定小鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、三酰甘油(TG)的水平及肝组织中还原型谷胱甘肽(GSH)和丙二醛(MDA)含量,并观察肝组织病理,进行组织病理学评分。结果与空白对照组比较,模型组小鼠血清ALT、AST水平及急性酒精性肝损伤中TG含量升高,肝脏中的MDA水平上升,GSH活性降低,差异有统计学意义(P<0.05)。与模型组比较,SCL给药组能够明显降低小鼠血清中ALT和AST水平及急性酒精性肝损伤中TG含量,抑制肝脏中上升的MDA水平,增强GSH的活性,差异均有统计学意义(P<0.05)。HE结果显示,模型组有明显的肝损伤,表现为细胞坏死、相邻肝细胞间的脂肪变性和淋巴细胞的渗透,而SCL高剂量组没有观察到严重的脂肪变性,肝脏损伤和肝结构的分裂程度最低。结论 SCL对小鼠急性酒精肝损伤具有保护作用,其可能是通过改善脂质过氧化而发挥作用。
Objective To study the protective effect of steamed Codonopsis lanceolata (SCL) on mice with acute hepatic injury induced by ethanol exposure. Methods Male ICR mice were randomly divided into 6 groups: blank control group, model group, silymarin group and SCL low, medium and high dose groups. In addition to the blank control group, the rest of the group to establish a mouse model of acute alcoholic liver injury. Each group to give the appropriate interventions. The contents of ALT, AST, TG and the levels of glutathione (GSH) and malondialdehyde (MDA) in liver were measured by colorimetric method. The pathological changes of liver were observed and histopathological scores were evaluated. Results Compared with the blank control group, the levels of serum ALT and AST, the content of TG in acute alcoholic liver injury increased, the level of MDA in liver increased and the activity of GSH decreased in model group (P <0.05). Compared with the model group, SCL administration group could significantly reduce the level of ALT and AST in serum, the content of TG in acute alcoholic liver injury, the level of MDA in the liver and the activity of GSH (the difference was statistically significant ( P <0.05). The results of HE showed that the model group had obvious hepatic injury characterized by necrosis of cells, steatosis of adjacent hepatocytes and infiltration of lymphocytes, while severe steatosis, liver damage and liver structure were not observed in high-dose SCL group The lowest degree of division. Conclusion SCL has a protective effect on acute alcoholic liver injury in mice, which may play a role by improving lipid peroxidation.