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目的探讨基因工程人血管内皮抑制素（ｅｎｄｏｓｔａｔｉｎ）抑制黑素瘤在小鼠体内生长和转移的作用及其作用机制。方法接种黑素瘤细胞悬液（２ × １０６／鼠）于小鼠皮下，成瘤后给予血管内皮抑制素（８ ｍｇ／ｋｇ· ｄ），每天 １次，共２１次。给药期间观察荷瘤小鼠的饮食情况、体重变化，并测量肿瘤的大小。于第２６天处死，称皮下肿瘤的重量，并取脑、肺、肝、脾和肾切片做病理学检查。结果血管内皮抑制素治疗组的曲线下面积明显小于肿瘤对照组（Ｐ＜０．０１）。病理切片检查显示，治疗组肿瘤有大面积的坏死，瘤周毛细血管消失。结论血管内皮抑制素对小鼠黑素瘤的生长、转移，以及新生毛细血管的形成具有明显地抑制作用。
Objective To investigate the effect of genetically engineered human endostatin on the growth and metastasis of melanoma in mice and its mechanism. Methods The melanoma cell suspension (2 × 106/mouse) was inoculated subcutaneously in mice and endostatin (8 mg/kg·d) was given after tumor formation, once a day for 21 times. During the administration period, the dietary status, body weight change of the tumor-bearing mice were observed, and the tumor size was measured. On the 26th day, he was sacrificed and the weight of the subcutaneous tumor was measured. Brain, lung, liver, spleen and kidney sections were taken for pathological examination. Results The area under the curve of the endostatin treatment group was significantly smaller than that of the tumor control group (P<0.01). Pathological examination showed that there was a large area of necrosis in the treated group, and the capillaries around the tumor disappeared. CONCLUSION: Endostatin can significantly inhibit the growth and metastasis of melanoma in mice and the formation of new capillaries.