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目的探讨磷酸钠对三价砷所致细胞膜损伤的保护作用。方法用NaAsO2处理非洲绿猴肾上皮细胞(Vero)后,在培养液中加入不同剂量磷酸钠剂,采用分光光度法分别测定细胞膜Na+,K+-ATP酶活性,高效液相法测定细胞膜磷脂组分含量改变。结果 2.20mg/L剂量的砷暴露可明显抑制Vero细胞膜Na+,K+-ATP酶活性;高剂量磷酸钠(69.00 mg/L)干预后,Na+,K+-ATP酶活性为(0.761±0.067)U/(mgprot.h)高于砷染毒组(0.544±0.037)U/(mgprot.h)(P<0.05);69.00 mg/L磷酸钠组细胞膜磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰丝氨酸及神经鞘磷脂含量分别为(0.506±0.073),(0.101±0.237),(0.083±0.005)和(0.066±0.003)mg/mL,明显高于砷染毒组(P<0.05)。结论一定剂量磷酸钠对砷染毒细胞的细胞膜Na+,K+-ATP酶活性及磷脂组分的改变具有保护作用。
Objective To investigate the protective effects of sodium phosphate on cell membrane damage induced by trivalent arsenic. Methods After treatment of Vero cells with NaAsO2, different dosage of sodium phosphate was added to the culture medium. The activity of Na + and K + -ATPase in the cell membrane was measured by spectrophotometry. The phospholipid fraction Change in content. Results Arsenic exposure at 2.20mg / L could significantly inhibit Na +, K + -ATPase activity in Vero cell membrane. The Na +, K + -ATPase activity was (0.761 ± 0.067) U / (mgprot.h) was higher than that of the arsenic-exposed group (0.544 ± 0.037) U / (mgprot.h) (P <0.05). The contents of phosphatidylcholine, phosphatidylethanolamine, The content of sphingomyelin was (0.506 ± 0.073), (0.101 ± 0.237), (0.083 ± 0.005) and (0.066 ± 0.003) mg / mL, respectively, which was significantly higher than that of arsenite exposure group (P <0.05). Conclusion A certain dose of sodium phosphate has a protective effect on the membrane Na +, K + -ATPase activity and phospholipid component change in arsenite-exposed cells.