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目的：了解CML急变期的TCRVβ亚家族T细胞的表达及其克隆性。方法：采用RT－PCR扩增4例CML急变期病人的外周血单个核细胞的TCRVβ24个亚家族的互补决定区3（CDR3），产物进一步经基因扫描分析确定T细胞的克隆性。结果：病人外周血仅表达4～9个Vβ亚家族T细胞，主要为Vβ1，Vβ2，Vβ3，Vβ5，Vβ15和Vβ17；基因扫描分析显示2例CML急粒变的部分产物为寡克隆性，而2例CML急淋变者的产物均为多克隆性。结论：CML急变期外周血仅选择性表达部分Vβ亚家族T细胞；CML急粒变存在克隆性增殖T细胞，这可能是机体对白血病细胞相关抗原的一种直接反应。该方法可用于检测微小残留病变和判断疾病复发。
Objective: To investigate the expression and clonality of TCR Vβ subfamily T cells in the blast crisis of CML. METHODS: The complementarity determining region 3 (CDR3) of TCR Vβ 24 subfamilies of peripheral blood mononuclear cells in 4 patients with CML blast crisis were amplified by RT-PCR. The clonality of T cells was further confirmed by gene scanning analysis. Results: The patient’s peripheral blood only expressed 4-9 Vβ subfamily T cells, mainly Vβ1, Vβ2, Vβ3, Vβ5, Vβ15 and Vβ17; gene scan analysis showed that 2 cases of CML telomere partial products were oligoclonal, and The products of two cases of CML acute lysate were polyclonal. CONCLUSION: The peripheral blood of CML is only selectively expressing part of Vβ subfamily T cells; CML has clonally proliferating T cells, which may be a direct response of the body to leukemia cell-associated antigens. This method can be used to detect minimal residual disease and determine disease recurrence.