用一阶导数分光光度法消除杂质成分对吸收度的影响,应用多波长数据线性回归法处理测定数据,不经分离,同时测定复方双氯灭痛注射液中的双氯灭痛和扑热息痛的含量,取得满意结果。测定波长为306,296,295,294,293及275nm。双氯灭痛和扑热息痛的平均回收率分别为100.89%(RSD=0.55%,n=6),99.33%(RSD=0.52%,n=6)。 The first-order derivative spectrophotometry was used to eliminate the effect of impurities on the absorbance. The multi-wavelength data linear regression method was used to treat the measured data. The content of diclofenac and paracetamol in the compound diclofenac injection was determined simultaneously without separation. Satisfactory results were obtained. The measurement wavelengths were 306, 296, 295, 294, 293 and 275 nm. The average recoveries of diclofenac and paracetamol were 100.89% (RSD = 0.55%, n = 6) and 99.33% (RSD = 0.52%, n = 6), respectively.