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土耳其斯坦筋骨草[Ajuga turkestanica(Rg1.)Brig(唇形科)]干叶经甲醇提取浓缩后用水稀释,己烷抽提,水层再用乙酸乙酯尽提得干固体提取物,经氧化铝柱纯化,用氯仿,氯仿-甲醇(9:1)洗脱,再将部分纯化物通过硅胶柱再层析,用2、3、4、5和10%甲醇-氯仿洗脱,依次得筋骨草内酯(Ajugalactone)、筋骨草内酯-β(Ajugalactone-B),cyansterone,蜕皮素-1(Ecdyson-Ⅰ),继续洗脱得β-蜕皮素(Ecdysterone)。蜕皮素-Ⅰ为新的化合物,产率为干生药的0.05%,C_(31)H_(46)O_9,熔点212～215°(丙酮-己烧),[α]_D~(29)+96.0±2°,Rf0.35(溶
Ajuga turkestanica (Rg1.) Brig] The dried leaves are extracted by methanol, concentrated, diluted with water and extracted with hexane. The aqueous layer is further extracted with ethyl acetate to give a dry solid extract, which is oxidized The column was purified by aluminum column, eluted with chloroform and chloroform-methanol (9: 1). The partially purified material was chromatographed on a silica gel column and eluted with 2,3,4,5 and 10% methanol-chloroform Ajugalactone, Ajugalactone-B, Cyansterone and Ecdyson-I, and continue to elute Ecdysterone. Ecdysone-Ⅰ was a new compound with the yield of 0.05%, C_ (31) H_ (46) O_9, melting point of 212 ~ 215 ° (acetone-hexane), [α] ± 2 °, Rf0.35 (dissolved