Objective To construct a subtracted cDNA library of hyperlipidemia-sensitive rabbits’ liver by suppression subtractive hybridization (SSH). Methods Tlie mRNAs were extracted from hyperlipidemia-sensitive and hyperlipidemia-insensitive rabbit liver respectively and converted into double-strand cDNAs. After digestion with restriction enzyme, the cDNA of hyperlipidemia-sensitive group was subdivided into two portions and each one was ligated with different adaptors. The differentially expressed cDNA were obtained by two rounds of both hybridization and suppression PCR. The PCR products were inserted into T/A vector to set up the subtractive cDNA library. The clones-selecting were amplified by PCR and identified. Results The amplified library contained 500 positive bacteria clones, including 463 clones which had an inserts from 250 to 700 bp PCR analysis. Conclusion A subtracted cDNA library of differentially expressed genes in hyperlipidemia-sensitive rabbit liver was constructed successfully by SSH and T/ Objective To construct a subtracted cDNA library of hyperlipidemia-sensitive rabbits’ liver by suppression subtractive hybridization (SSH). Methods Tlie mRNAs were extracted from hyperlipidemia-sensitive and hyperlipidemia-insensitive rabbit liver respectively and converted into double-strand cDNAs. After digestion with restriction The cDNA of hyperlipidemia-sensitive group was subdivided into two portions and each one was ligated with different adapters. The differentially expressed cDNA were obtained by two rounds of both hybridization and suppression PCR. The PCR products were inserted into T / A vector to Set the the subtractive cDNA library. The clones-selecting were amplified by PCR and identified. Results The amplified library contained 500 positive bacteria clones, including 463 clones which had an inserts from 250 to 700 bp PCR analysis. Conclusion A subtracted cDNA library of differentially expressed genes in hyperlipidemia-sensitive rabbit liver was constructed successf ully by SSH and T /