论文部分内容阅读
[Objective] To establish the fingerprint chromatograms of RADIX ZANTHOXYLI from Guangxi area in China by liquid chromatography-mass spectrometry (LC-MS). [Method] The seperation was performed on an Agilent Zorbax Eclipse C8 column(2.1 mm × 100 mm,3.5 μm)with the mobile phase consisting of methanol-acetonitrile-0.5% formic acid(adjusted to pH=4.5 with ammonia)in a gradient mode. The chromatograms were recorded by using a triple quadrupole mass spectrometer equipped with an ESI source in positive ion mode. Totally,15 batches of RADIX ZANTHOXYLI samples were analyzed and their similarities were calculated using fingerprint similarity evaluation software. [Results] The LC-MS fingerprint common model of RADIX ZANTHOXYLI produced in Guangxi area was obtained. A total of 22 characteristic peaks were selected as the common fingerprint peaks. The similarity between the 15 samples and the common model was above 0.9. [Conclusion] The reported method was precise,reproducible and reliable. The establishment of LC/MS fingerprint chromatograms might be helpful for the quality control of RADIX ZANTHOXYLI produced in Guangxi area.
[Objective] To establish the fingerprint chromatograms of RADIX ZANTHOXYLI from Guangxi area in China by liquid chromatography-mass spectrometry (LC-MS). [Method] The seperation was performed on an Agilent Zorbax Eclipse C8 column (2.1 mm × 100 mm, 3.5 μm) with the mobile phase consisting of methanol-acetonitrile-0.5% formic acid (adjusted to pH = 4.5 with ammonia) in a gradient mode. The chromatograms were recorded by using a triple quadrupole mass spectrometer equipped with an ESI source in positive ion mode Totally, 15 batches of RADIX ZANTHOXYLI samples were analyzed and their similarities were calculated using fingerprint similarity evaluation software. [Results] The LC-MS fingerprint common model of RADIX ZANTHOXYLI produced in Guangxi area was obtained. A total of 22 characteristic peaks were selected The similarity between the 15 samples and the common model was above 0.9. [Conclusion] The reported method was precise, reproducible and reliable. The es tablishment of LC / MS fingerprint chromatograms might be helpful for the quality control of RADIX ZANTHOXYLI produced in Guangxi area.