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采用聚合酶链式反应(PCR)技术,以阜平大枣枣缩果病果实病部、阜平大枣健康果实抽提到的组织总DNA为模板,对其细菌16S rDNA的V3可变区及真菌18S rDNA进行扩增,并对扩增产物进行DGGE指纹图谱分析,研究阜平大枣缩果病果实与健康果实内的微生物种群变化。试验结果表明,PCR-DGGE法是研究枣果内微生物组成的可行方法;阜平大枣缩果病果实内细菌及真菌菌群种类较健康果实减少,有些细菌及真菌的优势菌在病果样品内不存在,健康样品内的某些优势菌在病部为非优势菌。
Polymerase chain reaction (PCR) technique was used to detect the changes of the 16S rDNA V3 variable region of Fuping jujube jujube fruit and the total tissue DNA extracted from healthy fruits of Fuping jujube as template. And fungus 18S rDNA amplification, and DGGE fingerprinting analysis of the amplified products to study the jujube fruit shrinkage Piping fruits and healthy fruit within the microbial population changes. The results showed that the PCR-DGGE method is a feasible method to study the composition of microorganisms in jujube fruit. The species of bacteria and fungi in fruit of jujube fruit in Fuping decreased compared with those in healthy fruits. Some dominant bacteria of bacteria and fungi in the fruit samples Does not exist within the healthy samples of certain dominant bacteria in the disease is non-dominant bacteria.