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本研究应用SSR和SRAP 2种标记对11个苦瓜品种进行鉴定和亲缘关系分析。结果发现,应用筛选的8对SSR引物共扩增条带860条,多态性条带占68.0%,10对SRAP引物共扩增条带961条,多态性条带占79.4%。SSR标记检测到品种间遗传相似系数介于0.344~0.779,平均值为0.652;SRAP标记检测到品种间遗传相似系数介于0.373~0.700,平均值为0.625。聚类分结果发现,在遗传相似系数为0.54和0.52时,SSR和SRAP 2种标记都可以将11个品种分为相同的两组。每对SSR和SRAP引物能平均区分3.1和3.7个品种。最少用3对SSR引物或3对SRAP引物组合就可成功区分11个苦瓜品种。研究结果表明,SSR和SRAP标记均可高效地被用于苦瓜的品种鉴定和亲缘关系分析。
In this study, 11 bitter melon cultivars were identified and their genetic relationship analyzed by SSR and SRAP markers. The results showed that 860 SSR primers were used to amplify a total of 860 bands, 68.0% of which were polymorphic bands. A total of 961 bands were amplified by 10 pairs of SRAP primers and 79.4% of them were polymorphic bands. The SSR markers showed that the genetic similarity coefficients of the cultivars ranged from 0.344 to 0.779 with an average of 0.652. The genetic similarity coefficients detected by SRAP markers ranged from 0.373 to 0.700 with an average of 0.625. Cluster analysis showed that SSR and SRAP markers could divide 11 cultivars into the same two groups with genetic similarity coefficients of 0.54 and 0.52. Each pair of SSR and SRAP primers evenly discriminates between 3.1 and 3.7 varieties. A minimum of 11 pairs of SSR primers or 3 pairs of SRAP primer combinations were successfully used to distinguish 11 bitter melon varieties. The results showed that both SSR and SRAP markers could be effectively used to identify the species and genetic relationship of bitter gourd.