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目的:通过建立大鼠孕期营养性铁缺乏模型,探讨母鼠孕期低铁干预对其子鼠TNF-α,IL-6,EPO的影响。方法:将6周龄健康Wistar雌性大鼠随机分为二组,实验组喂低铁饲料,对照组喂富铁饲料。4周后与健康成年Wistar雄鼠同笼交配,低铁干预持续至哺乳期结束,随后二组大鼠均转入铁平衡期,持续7周。将实验组和对照组各自再分为两小实验组,每小实验组再经低铁干预。持续14周后宰杀大鼠,检测仔鼠血浆中TNF-α,IL-6,EPO的水平。结果:低铁干预组子代大鼠体重在断乳以后及转入平衡期饲喂富铁饲料后比对照组增长显著,呈现追赶生长;母鼠孕期低铁干预,其子鼠成年后再次铁干预,实验组血浆中TNF-α,EPO和IL-6均高于对照组(P<0.05)。结论:母鼠孕期低铁干预,其子鼠的体重在断乳后及补铁后生长迅速并显著超过对照组;铁缺乏可改变体内细胞因子的水平;生命早期低铁干预可产生更大的影响,并由此可能影响由该细胞因子介导的免疫反应。
OBJECTIVE: To investigate the effect of low iron intervention during pregnancy on the levels of TNF-α, IL-6 and EPO in their offsprings by establishing a model of nutritional iron deficiency during pregnancy in rats. Methods: Six-week-old healthy Wistar female rats were randomly divided into two groups. The experimental group was fed with low-iron diet and the control group fed with iron-rich diet. Four weeks later, males were mated with healthy adult Wistar male rats. Low-iron intervention lasted until the end of lactation, and then both rats were transferred to iron balance period for 7 weeks. The experimental group and control group were divided into two small experimental groups, each small experimental group and then by low-iron intervention. After 14 weeks, the rats were sacrificed and the levels of TNF-α, IL-6 and EPO in the plasma of the pups were detected. Results: The body weight of offspring rats in low-iron intervention group increased significantly compared with the control group after weaning and fed the iron-rich diet, Intervention, the experimental group plasma TNF-α, EPO and IL-6 were higher than the control group (P <0.05). Conclusion: During low iron intervention in pregnant rats during pregnancy, the weight of their offspring grew rapidly after weaning and iron supplementation and significantly exceeded that of the control group. Iron deficiency could change the level of cytokines in vivo. Low iron intervention in early life could produce greater Affect, and thus may affect, the immune response mediated by the cytokine.