论文部分内容阅读
目的研究肺结核患儿与卡介苗接种抗体阳性儿童外周血TCR Vβ基因克隆。方法分别提取15名肺结核患儿及15名卡介苗接种抗体阳性儿童外周血RNA,经逆转录扩增后,以Genescan方法分别检测其TCR Vβ的基因克隆。以健康儿童卡介苗接种抗体阴性作为对照。结果肺结核患儿与卡介苗接种抗体阳性儿童外周血TCR Vβ基因家族均以Vβ2,Vβ16,Vβ21,Vβ22克隆增生为主,克隆抑制以Vβ14和Vβ17为主;不同的个体之间TCR Vβ基因克隆不尽相同。结论卡介苗接种抗体阳性儿童与肺结核患儿具有相似的TCR TCR Vβ基因克隆增生家族。
Objective To study the cloning of TCR Vβ gene in children with tuberculosis and BCG immunization. Methods Peripheral blood RNA was extracted from 15 children with tuberculosis and 15 from patients with BCG vaccination. After reverse transcriptase amplification, the TCR Vβ gene clones were detected by Genescan method. Take healthy children BCG vaccination antibody negative as a control. Results The TCR Vβ gene family in children with tuberculosis and BCG vaccination antibody positive were mainly Vβ2, Vβ16, Vβ21 and Vβ22 clonal proliferation, while Vβ14 and Vβ17 were predominant in clonal suppression. TCR Vβ gene clones were not found in different individuals the same. CONCLUSIONS: BCG vaccination-positive children have similar TCR TCR Vβ gene clonal proliferation family with children with tuberculosis.