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目的研究酪氨酸蛋白激酶抑制剂槲皮素对ＰＤＧＦ诱导的ＮＩＨ３Ｔ３细胞增殖的影响。方法采用ＭＴＴ比色法、Ｇｉｅｍｓａ染色、流式细胞术（测定ＤＮＡ含量的变化及增殖细胞核抗原的表达）、及ＷｅｓｔｅｒｎＢｌｏｔ分析技术对ＰＤＧＦ诱导的ＮＩＨ３Ｔ３细胞增殖进行分析。结果与对照组相比，槲皮素处理可显著地抑制ＰＤＧＦ诱导的ＮＩＨ３Ｔ３细胞增殖，而且主要是细胞周期Ｓ期抑制，ＷｅｓｅｔｅｒｎＢｌｏｔ分析提示槲皮素可明显地抑制ＰＤＧＦ诱导的ＮＩＨ３Ｔ３细胞酪氨酸磷酸化程度。结论酪氨酸蛋白激酶抑制剂可显著地抑制ＰＤＧＦ诱导的ＮＩＨ３Ｔ３细胞增殖，可能是通过抑制酪氨酸蛋白激酶活性来完成。
Objective To investigate the effect of tyrosine kinase inhibitor quercetin on PDGF-induced NIH3T3 cell proliferation. Methods MTT assay, Giemsa staining, flow cytometry (determination of DNA content and expression of proliferating cell nuclear antigen), and Western Blot analysis of PDGF-induced NIH3T3 cell proliferation were analyzed. Results Compared with the control group, quercetin treatment could significantly inhibit PDGF-induced proliferation of NIH3T3 cells and mainly inhibit the cell cycle S phase. WeseternBlot analysis suggested that quercetin significantly inhibited tyrosine phosphorylation of NIH3T3 cells induced by PDGF Degree of. Conclusion Tyrosine kinase inhibitor can significantly inhibit PDGF-induced NIH3T3 cell proliferation, probably by inhibiting tyrosine kinase activity.