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目的:取肾癌病人标本建立一株新的人肾癌细胞系,初步对该细胞系进行鉴定,为进一步的肾癌基础研究提供实验模型。方法:2008-2009年间共采集20例肾癌新鲜手术标本,于手术后1h内将每例标本切取四块0.5cm×0.5cm×0.5cm组织块,分别包埋于2只裸鼠的右后肢皮下及背部皮下,连续传代3次,取移植瘤体外培养,记录细胞株的生长曲线,测定细胞集落形成率,对细胞进行DNA含量测定,进行染色体分析及病理学检查。结果:其中1只裸鼠皮下移植瘤生长,继续传代,肿瘤生长速度明显加快。取移植瘤标本体外培养得到肾癌细胞系XJG-9201。形态结构,分化程度与原发瘤一致,染色体众数为65。细胞群体倍增时间为38.2h,细胞周期分析G1期62.7%,G2期11.2%,S1期25.3%,集落形成率为70%。结论:肾癌细胞系XJG-9201与原发癌保持相同的生物学特性,体外连续传代1年以上传115代。细胞形态不变,生长周期恒定,已成为一个稳定的细胞系。
OBJECTIVE: To establish a new human renal cell carcinoma cell line from patients with renal cell carcinoma and to identify the cell line initially, providing an experimental model for further basic research of renal cell carcinoma. Methods: A total of 20 fresh surgical specimens of kidney cancer were collected during 2008-2009. Four specimens of 0.5cm × 0.5cm × 0.5cm tissue were excised from each specimen within 1h after operation and were embedded in the right hind limbs of 2 nude mice respectively Subcutaneously and dorsally subcutaneously for 3 times in succession. The transplanted tumor was cultured in vitro. The growth curve of the cell line was recorded. The colony formation rate was measured. The DNA content of the cells was measured, and the chromosome analysis and pathological examination were performed. Results: One of the nude mice grew subcutaneously and continued to pass the tumor. The tumor growth rate was significantly accelerated. Take the xenograft specimens cultured in vitro to obtain renal cell carcinoma cell line XJG-9201. Morphology and structure, the degree of differentiation consistent with the primary tumor, chromosome mode of 65. Cell population doubling time was 38.2 h, cell cycle analysis of G1 phase 62.7%, G2 11.2%, S1 25.3%, colony formation rate of 70%. Conclusion: The renal cell carcinoma cell line XJG-9201 has the same biological characteristics as the primary carcinoma, and passaged for more than one year in vitro for 115 passages. Cell shape unchanged, constant growth cycle, has become a stable cell line.