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目的观察日本血吸虫云南品系未成熟卵可溶性抗原SIEA免疫小鼠后攻击感染皖鄂品系血吸虫尾蚴,是否也能诱导出免疫保护效果。方法 20只ICR小鼠随机分为免疫组和对照组,经日本血吸虫云南品系SIEA免疫后攻击感染皖鄂品系血吸虫尾蚴,感染后46 d观察减虫率、粪卵减少率、雌虫子宫内虫卵数、肝表面虫卵结节数、肝脏各期虫卵数及各期虫卵构成比。结果 SIEA免疫后粪卵及雌虫子宫内虫卵减少率分别为34.45%,23.69%(P<0.05);肝表面虫卵结节密度下降29.03%(P<0.05);肝组织内虫卵减少29.07%(P<0.05),成熟虫卵减少48.41%(P<0.05)。肝组织内成熟虫卵比例下降,未成熟卵比例增加(P<0.05)。结论日本血吸虫(云南品系)未成熟卵可溶性抗原(SIEA)的抗原性较强,小鼠经云南品系SIEA免疫后攻击感染皖鄂品系血吸虫尾蚴也诱导小鼠产生了抗卵胚发育及抗雌虫生殖免疫力,结果提示将来在中国大陆使用SIEA疫苗时,似可不必考虑血吸虫品系的影响。
Objective To observe the immune protection effect of Schistosoma japonicum Yunnan strain immature egg-soluble antigen SIEA immunized mice after challenge infected with Schistosoma japonicum Echinococcus cercariae. Methods Twenty ICR mice were randomly divided into immunized group and control group. After immunization with Schistosoma japonicum Yunnan strain SIEA, Schistosoma japonicum cercariae were challenged. After 46 days, the worm reduction rate, fecal egg reduction rate, The number of eggs, the number of eggs on the surface of the liver, the number of eggs in each stage of the liver, and the composition ratio of each stage of eggs. Results The rates of reduction of eggs in feces and females after immunization with SIEA were 34.45% and 23.69%, respectively (P <0.05). The density of eggs on the surface of liver decreased by 29.03% (P <0.05) 29.07% (P <0.05), mature egg decreased 48.41% (P <0.05). The proportion of mature eggs in liver tissue decreased, the proportion of immature eggs increased (P <0.05). Conclusions The antigenicity of immature egg-soluble antigen (SIEA) of Schistosoma japonicum (Yunnan strain) is strong. Mice infected with SIEA of Yunnan strain SJA-infected Echovirus strain also induced the development of anti-egg embryo Reproductive immunity, the results suggest that future use of SIEA vaccine in mainland China, it may not have to consider the impact of schistosomiasis strains.