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目的观察补肾调冲方对卵巢早衰模型大鼠血清促卵泡刺激素(FSH)、促黄体生成素(LH)、抗缪勒管激素(AMH)水平及卵巢组织肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)蛋白表达的影响。方法 60只SD雌性大鼠随机分为6组:对照组,模型组,补肾调冲方低、中、高剂量(以生药计24、12、6 g/kg)组,结合雌激素片(阳性药)组,每组10只。除对照组外,其余各组均ig雷公藤多苷片造模14 d,造模结束24 h后,给予相应药物治疗36 d。给药结束后,酶联免疫吸附(Elisa)法检测大鼠血清FSH、LH、AMH水平,免疫组化法检测卵巢组织TNF-α、IFN-γ蛋白表达。结果与对照组比较,模型组血清FSH、LH水平、卵巢TNF-α、IFN-γ蛋白表达均显著升高,AMH水平显著降低(P<0.05);与模型组比较,各给药组FSH、LH水平、卵巢TNF-α、IFN-γ蛋白表达均显著降低,AMH水平显著升高(P<0.01)。结论补肾调冲方可提高卵巢早衰大鼠卵巢储备能力,抑制卵泡封闭及黄体细胞凋亡,对卵巢早衰可能有治疗作用。
Objective To observe the effect of Bushen Tiaochong Recipe on serum levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), anti-mullose hormone (AMH) and tumor necrosis factor-α (TNF- , Interferon-γ (IFN-γ) protein expression. Methods Sixty SD female rats were randomly divided into 6 groups: control group, model group, Bushen Tiaochong Recipe at low, medium and high doses (24,12,6 g / kg as crude drug), combined with estrogen tablets ) Group, 10 in each group. In addition to the control group, the rest of the groups were ig triploid glycosides tablet modeling 14 d, 24 h after the end of modeling, the corresponding drug treatment for 36 d. Serum levels of FSH, LH and AMH were detected by Elisa method and the expressions of TNF-α and IFN-γ in ovarian tissue were detected by immunohistochemistry. Results Compared with the control group, the serum levels of FSH and LH, the levels of TNF-α and IFN-γ in ovary increased significantly and the levels of AMH decreased significantly (P <0.05). Compared with the model group, LH, ovarian TNF-α, IFN-γ protein expression were significantly lower, AMH levels were significantly increased (P <0.01). Conclusion Bushen Tiaochong can improve ovarian reserve in rats with premature ovarian failure, inhibit the closure of follicles and apoptosis of luteal cells and may have a therapeutic effect on premature ovarian failure.