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目的 了解中、重度急性低压缺氧对大鼠血液、心肌、脑皮质一氧化氮分泌的影响及红景天药物干预情况,进而探讨不同程度缺氧时红景天对大鼠不同部位一氧化氮调节的异同。方法 36只健康雄性SD大鼠,随机分成盐水组和红景天药物组,每组又分作地面、5000m、8000m高空低压组。盐水组、红景天组每天分别灌胃生理盐水、红景天浓缩液,共7d。采用721型分光光度计,以硝酸还原酶法检测地面组和低压暴露5h后缺氧组血浆、心肌及脑组织一氧化氮含量。结果 5000,8000m急性缺氧盐水组与地面盐水组比较,血浆、心肌、脑组织一氧化氮含量降低(P<0.05或P<0.01)。5000,8000m急性缺氧红景天组与相应盐水组相比,血浆、心肌、脑组织一氧化氮含量升高(P<0.05或P<0.01)。地面红景天组与地面盐水组相比,血浆一氧化氮含量的差异无显著性意义。结论 在正常状态下红景天对机体一氧化氮水平无影响。在中、重度急性低压缺氧时红景天可以升高机体异常降低的一氧化氮水平。
Objective To investigate the effects of moderate and severe acute hypobaric hypoxia on the secretion of nitric oxide in rat blood, myocardium and cerebral cortex and drug intervention of Rhodiola, and to investigate the effects of different doses of Rhodiola on the nitric oxide in different parts of rats. Similarities and differences in regulation. Methods Thirty-six healthy male Sprague-Dawley rats were randomly divided into saline group and Rhodiola drug group. Each group was divided into high altitude and low pressure groups with altitude of 5000m and 8000m. The saline group and rhodiola group were given normal saline and Rhodiola daily for 7 days. Using a 721 spectrophotometer, nitric oxide levels in plasma, myocardium, and brain tissue in the hypoxia group were detected by the nitrate reductase method in the ground and 5-hour exposure to low pressure. Results The levels of nitric oxide in plasma, myocardium and brain tissue were decreased in the 5000 and 8000m acute hypoxic saline group compared with the saline group (P<0.05 or P<0.01). In the 5000 and 8000m acute hypoxia rhodiola group, the levels of nitric oxide in plasma, myocardium and brain tissue were significantly higher than those in the corresponding saline group (P<0.05 or P<0.01). There was no significant difference in plasma nitric oxide levels between the ground salidroside group and the saline group. Conclusion Rhodiola had no effect on the level of nitric oxide in the body under normal conditions. Rhodiola can increase abnormally reduced levels of nitric oxide in moderately severe acute hypobaric hypoxia.