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目的对老年T2DM患者及健康人群尿液的多肽谱进行定量和定性分析,探讨老年T2DM患者与健康人群的标志性蛋白。方法取20例老年T2DM患者(老年T2DM组)和22名健康人(NC组)尿样,富集尿液中的多肽,并进行在线反相液相色谱分离和串联质谱分析,检测尿液中的多肽谱。获得的原始质谱图导入到ProteinPilot软件(v4.5)行数据库检索比对分析,以鉴定蛋白质。结果老年T2DM组尿液中测得816条多肽,对应100种蛋白;NC组尿液中测得1048条多肽,对应133种蛋白。两组尿液多肽含量和种类不同,对应的蛋白也不同。老年T2DM组尿液较NC组增加的多肽对应的蛋白为转甲状腺素蛋白(TTR)、载脂蛋白A-1(ApoA-1)、.ApoA-4、骨桥蛋白(OPN)及补体C3。结论尿液中的TTR、ApoA-1、ApoA-4、OPN及补体C3可能是区分老年T2DM患者和健康人群的标志性蛋白。
Objective To quantitative and qualitative analysis of urine peptide profiles in elderly patients with T2DM and healthy subjects to explore the landmark proteins in elderly patients with T2DM and healthy people. Methods Urine samples of 20 elderly patients with T2DM (T2DM elderly) and 22 healthy subjects (NC) were collected and the urine peptides were enriched and analyzed by on-line reversed-phase liquid chromatography and tandem mass spectrometry Peptide profile. The original spectra obtained were imported into a ProteinPilot software (v4.5) row database for alignment analysis to identify proteins. Results In the elderly T2DM group, 816 peptides were detected in urine, which corresponded to 100 proteins. In the urine of NC group, 1048 peptides were detected in urine, corresponding to 133 proteins. Two groups of different levels and types of urine peptides, the corresponding protein is also different. Urine in the elderly T2DM group compared with the NC group increased peptide TTR, ApoA-1, ApoA-4, osteopontin (OPN) and complement C3. Conclusion TTR, ApoA-1, ApoA-4, OPN and complement C3 in urine may be the hallmarks of differentiating elderly patients with T2DM and healthy people.