目的探讨维生素E抗肝纤维化作用的机理。方法采用胶原酶、链蛋白酶E消化及密度梯度离心的方法 ,分离、培养大鼠肝星状细胞 ,用四甲基偶氮唑蓝 (MTT)比色法及3 H 脯氨酸掺入法 ,观察维生素E对星状细胞增殖及胶原合成的影响。结果低剂量维生素E对肝星状细胞及胶原合成的抑制作用不明显 ,随着维生素E的浓度增加 ,对其抑制作用增强 ,呈明显的药物浓度依赖关系。结论维生素E抑制肝星状细胞增殖及胶原合成是其抗肝纤维化作用机理之一。 Objective To explore the mechanism of vitamin E against hepatic fibrosis. Methods Rat hepatic stellate cells were isolated and cultured by collagenase, trypsin E digestion and density gradient centrifugation. MTT assay and 3H-proline incorporation assay were used to detect the expression of Fas, To observe the effect of vitamin E on the proliferation and collagen synthesis of stellate cells. Results Low dose vitamin E had no obvious inhibitory effect on the synthesis of hepatic stellate cells and collagen. With the increase of vitamin E concentration, the inhibitory effect of vitamin E was enhanced, and the drug concentration dependence was obvious. Conclusion Vitamin E inhibits hepatic stellate cell proliferation and collagen synthesis is one of the mechanisms of anti-hepatic fibrosis.