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为探讨化脓性脑膜炎时地塞米松 (DEX)治疗与脑源性神经营养因子 (BDNF)的关系 ,以观察其神经保护作用。用大鼠建立化脓性脑膜炎模型 ,分别用抗生素及抗生素加DEX治疗 ,用免疫组化法检测脑组织BDNF的表达。结果 :感染后24h在脑组织和渗出到软脑膜、蛛网膜下腔、脑室及脑实质炎性病灶内的炎性细胞高水平表达BDNF蛋白。抗生素治疗后BDNF蛋白表达降到很低水平 ,与感染后24h组比较差异有显著性 (P<0.01) ,DEX组BDNF蛋白在大脑组织表达明显增多 ,与抗生素组比较差异有显著性 (P<0.01) ,但仍低于感染后24h组 (P<0.05)。提示DEX可能通过上调BDNF蛋白的表达 ,在化脓性脑膜炎时产生神经保护作用 ,而这一作用是有限的
To investigate the relationship between dexamethasone (DEX) treatment and brain-derived neurotrophic factor (BDNF) in purulent meningitis and to observe its neuroprotective effect. The model of purulent meningitis was established in rats and treated with antibiotics and antibiotics plus DEX respectively. The expression of BDNF in brain tissue was detected by immunohistochemistry. RESULTS: BDNF protein was expressed at high level in brain tissue and inflammatory cells exudation to the pia mater, subarachnoid space, ventricles and parenchymal inflammatory lesions 24 h after infection. BDNF protein expression dropped to a very low level after antibiotic treatment, which was significantly different from that of 24h group (P <0.01). The expression of BDNF protein in DEX group was significantly increased in the brain tissue compared with the antibiotic group (P < 0.01), but still lower than 24h after infection (P <0.05). These results suggest that DEX may have neuroprotective effects on purulent meningitis by up-regulating the expression of BDNF protein, and this effect is limited