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分别从印度萨瓦格拉姆班氏丝虫病流行区及昌迪加尔非流行区收集人体血标本,127份血清分为正常人(流行区和非流行区)、丝虫病(微丝蚴血症及临床丝虫病)及非丝虫病流行区肠道蠕虫感染(如钩、蛔虫感染)5组,按Kaliraj法制备兔抗微丝蚴血清(RAS),从丝虫性象皮肿和鞘膜积液病人收集血清,取50ml血以35%饱和硫酸铵沉淀免疫球蛋白(Ig),沉淀部分置3ml磷酸钠缓冲液(pH7.2,0.05M)透析过夜,获得人体丝虫血清免疫球蛋白(FSI),用DEAE-Sephadex-A50层析FSI(9ml磷酸钠缓冲液含蛋白810
Human blood samples were collected from the endemic areas of Banjara Swaziland and the non-endemic area of Chandigarh, respectively, and 127 sera were divided into normal (endemic and non-endemic), filariasis And clinical filariasis) and intestinal worm infection (such as hook and roundworm infection) in non-filariasis endemic areas. Rabbit anti-microfilaremia serum (RAS) was prepared according to the method of Kaliraj. Serum collected from patients with hydrocele, 50ml of blood to 35% saturated ammonium sulfate precipitation of immunoglobulin (Ig), the precipitate part of the home 3ml sodium phosphate buffer (pH 7.2, 0.05M) dialysis overnight to obtain human silkworm serum immunization Globulin (FSI), chromatographed with DEAE-Sephadex-A50 FSI (9 ml sodium phosphate buffer containing protein 810