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目的 :探讨谷氨酸神经毒性在不同脑区的差异。方法 :新生 SD大鼠 2 9只 ,生后第 3至第 9天 ,分为 1g/ kg、2 g/ kg、3g/ kg 3组 ,腹腔内注射谷氨酸单钠 ( monosodium glutamate,MSG) ,并设空白对照组和注射生理盐水组。 3月龄时取脑作石蜡切片 ,Spoerri氏法染色。光镜下计数丘脑、下丘脑、杏仁核、海马、齿状回、梨状皮质等 16个脑区的神经元数 ,并进行统计学分析。结果 :多数脑区注射 MSG后神经元减少 ,并与注射量呈正相关 ,但齿状回、尾 -壳复合体无明显变化 ,背侧内梨状核、杏仁内侧核、海马 CA3区和海马 CA4区的神经元 ,在低剂量组无明显减少 ,而在中、高剂量组明显减少。结论 :MSG对不同脑区的损伤程度不同 ,有些脑区具有某种保护机制 ,使神经元免受伤害
Objective: To investigate the differences of glutamate neurotoxicity in different brain regions. Methods: Twenty-nine newborn Sprague-Dawley rats were randomly divided into 3 groups (1g / kg, 2g / kg and 3g / kg). The mice were injected intraperitoneally with monosodium glutamate (MSG) , And set the blank control group and injected saline group. 3-month-old brain for paraffin section, Spoerri’s staining. Count the number of neurons in 16 brain regions including thalamus, hypothalamus, amygdala, hippocampus, dentate gyrus and piriform cortex under light microscope and make statistical analysis. Results: After injection of MSG, the number of neurons decreased in most brain regions and was positively correlated with the injection volume. However, there was no significant change in dentate gyrus and caudal - shell complex. In dorsal penis nucleus, medial almond, hippocampal CA3 region and hippocampal CA4 The neurons in the area showed no significant decrease in the low-dose group, but significantly reduced in the middle- and high-dose groups. Conclusion: MSG has different degrees of damage to different brain regions, and some brain regions have some protection mechanism to protect neurons from injury