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目的制备一种能提高T细胞对癌细胞杀伤能力的双特异性抗体。方法利用生物可降解材料PLGA(聚乳酸-羟基乙酸共聚物)制备成纳米颗粒,然后利用化学方法在纳米颗粒表面偶联能靶向结合T细胞的CD3抗体和癌细胞标志物MUC1的抗体,利用T细胞对癌细胞的杀伤实验来检测其功能。结果成功制备出直径200 nm的PLGA纳米颗粒并偶联上CD3和MUC1抗体,功能检测得出制备的双特异性抗体能显著提高T细胞对癌细胞的杀伤率。结论制备出一种能显著提高T细胞对癌细胞杀伤率的双特异性抗体。
Objective To prepare a bispecific antibody that can enhance the ability of T cells to kill cancer cells. Methods Nanoparticles were prepared by using biodegradable material PLGA (polylactide-co-glycolide), and then the antibodies capable of targeting CD3 antibody and cancer cell marker MUC1 were conjugated on the surface of nanoparticles. T cells on cancer cell killing experiments to test its function. Results The PLGA nanoparticles with a diameter of 200 nm were successfully prepared and conjugated with CD3 and MUC1 antibodies. The results showed that the prepared bispecific antibody could significantly increase the killing rate of T cells to cancer cells. Conclusion A bispecific antibody that can significantly increase the killing rate of T cells to cancer cells was prepared.