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目的:研究芍药甙对大鼠肝细胞体外损伤的防治作用。方法:采用D-氨基半乳糖和内毒素损伤大鼠原代培养肝细胞的方法造模,通过检测细胞形态变化和生化指标LDH、GOT、SOD等,观察芍药甙对其预防和治疗作用。结果:与各损伤组相对应的预防、治疗组,细胞表面结构、细胞核、内质网、高尔基复合体及线粒体、溶酶体等结构恢复正常。LDH、GOT、SOD也明显降低(P<0.05～0.01)。结论:芍药甙可通过增强肝细胞膜及溶酶体膜的稳定性,对肝细胞损伤起防治作用。
Objective: To study the preventive and therapeutic effects of Paeoniflorin on rat hepatocytes in vitro. METHODS: The rat primary cultured hepatocytes were injured by D-galactosamine and endotoxin. The morphological changes and biochemical indexes such as LDH, GOT and SOD were detected to observe the preventive and therapeutic effects of Paeoniflorin. Results: In the prevention and treatment groups corresponding to each injury group, the cell surface structure, nucleus, endoplasmic reticulum, Golgi complex, and mitochondria, lysosome structure returned to normal. LDH, GOT, and SOD also decreased significantly (P<0.05 to 0.01). Conclusion: Paeoniflorin can prevent and treat hepatocellular injury by enhancing the stability of hepatocyte membrane and lysosome membrane.