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目的建立同时测定芍苓消银片中芍药苷、落新妇苷、绿原酸、迷迭香酸和甘草酸含量的HPLC。方法采用Diamonsil C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈(A)-0.1%甲酸(B)系统,梯度洗脱;多种波长下同时检测。结果芍药苷、落新妇苷、绿原酸、迷迭香酸和甘草酸的保留时间依次为44.2,57.5,28.6,72.3,90.6 min,回归方程依次为:y=33 605 228x-124 483,r=0.999 9,线性范围为3.25~60.5μg·mL-1;y=101 354 460x-100 4,r=0.999 9,线性范围为6.5~130μg·mL-1;y=119 599 014x+22 521,r=1.000 0,线性范围为0.98~19.6μg·mL-1;y=104 333 261x-365 9,r=0.999 9,线性范围为0.75~15μg·mL-1;y=24 700 178x+7 185,r=0.999 9,线性范围为8~160μg·mL-1。平均加样回收率依次为98.7%,100.4%,99.6%,102.1%,102.3%,RSD依次为为2.17%,0.74%,2.64%,1.39%,1.93%。结论本方法操作较为简便,测定结果准确可靠,重复性好,能排除其他成分的干扰,可用于该制剂的质量控制。
OBJECTIVE To establish a HPLC method for simultaneous determination of paeoniflorin, astilbin, chlorogenic acid, rosmarinic acid and glycyrrhizic acid in Shaolingxiaoying tablet. Methods Diamonsil C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile (A) -0.1% formic acid (B) with gradient elution and simultaneous detection at various wavelengths. Results The retention times of paeoniflorin, astilbin, chlorogenic acid, rosmarinic acid and licorice acid were 44.2, 57.5, 28.6, 72.3 and 90.6 min, and the regression equations were as follows: y = 33 605 228x-124 483, r = 0.999 9, the linear range was 3.25-60.5 μg · mL-1, y = 101 354 460x-100 4, r = 0.999 9, the linear range was 6.5-130 μg · mL-1, y = 119 599 014x + 22 521, y = 104 333 261x-365 9, r = 0.999 9, linear range of 0.75-15 μg · mL-1; y = 24 700 178x + 7 185 , r = 0.999 9, linear range of 8 ~ 160μg · mL-1. The average recoveries were 98.7%, 100.4%, 99.6%, 102.1% and 102.3%, respectively. The RSDs were 2.17%, 0.74%, 2.64%, 1.39% and 1.93%, respectively. Conclusion The method is simple, accurate, reliable and reproducible. The method can eliminate the interference of other components and can be used for the quality control of the preparation.