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用籼稻品种澳青占、早丝占、七山占的幼胚和幼穗为外植体,探索以农杆菌为介导的GUS基因在籼稻中的转移,结果共培养后的愈伤组织比未经共培养的愈伤组织生长缓慢,再生植株的诱导条件亦有变化,共培养时间以40分钟至2小时为佳,卡那霉素选择浓度应大于200μg/ml。由GUS基因阳性反应的愈伤组织得到两株亦显阳性的植株,移入大田后,前期生长正常并开花,但开花之后死亡
Agropyron-mediated transfer of GUS gene in Indica rice was carried out using indica varieties Aoqingzhan, Zaisihan and Qishanzhan, as embryo explants. As a result, the callus ratio after co-cultivation The non-co-cultured callus grew slowly, and the induction conditions of the regenerated plants also varied. The co-cultivation time was preferably 40 minutes to 2 hours, and the selective concentration of kanamycin should be greater than 200 μg / ml. Two plants that were also positive were also found to be positive by the GUS gene callus. After they were transferred to the field, they grew normally in the early stage and flowered but died after flowering