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目的:对Real-time PCR技术在食物中毒中的应用进行初步研究。方法:采用高度特异、敏感的Real-time PCR作为初筛方法,GB方法同时进行细菌分离培养,用ATB全自动细菌鉴定仪对分离到的病原菌进行生化鉴定。结果:采用Real-time PCR检测22份剩余食物,其中6份检出副溶血性弧菌特异性DNA核酸;GB细菌培养法从这6份样品中检出3株副溶血性弧菌;其余样品两法均未检出副溶血性弧菌。所有样本均未检出沙门菌,志贺菌,单核细胞增生李斯特菌和大肠杆菌O157。结论:实时Real-time PCR检测食物中毒病原菌与GB培养法相比明显缩短检测周期,特异性强,敏感性高,在食物中毒事件中能更及时指明方向,起到快速初筛的作用,对临床治疗和食物中毒快速处置发挥积极作用,值得应用和推广。
Objective: To study the application of Real-time PCR technology in food poisoning. Methods: The highly specific and sensitive Real-time PCR was used as the primary screening method. The GB method was used to separate and culture the bacteria at the same time. The isolated pathogenic bacteria were biochemically identified by ATB automatic bacterial analyzer. Results: Twenty-two remaining samples were detected by Real-time PCR, of which six were Vibrio parahaemolyticus-specific DNA nucleic acids. Three strains of Vibrio parahaemolyticus were detected by GB bacterial culture from the six samples. The remaining samples Vibrio parahaemolyticus was not detected by either method. Salmonella, Shigella, Listeria monocytogenes and Escherichia coli O157 were not detected in all samples. Conclusion: The real-time real-time PCR detection of food poisoning pathogen significantly shortens the detection period compared with GB culture method, with high specificity and sensitivity. It can promptly identify the direction in food poisoning events and play a role in rapid screening. Treatment and rapid disposal of food poisoning play an active role, it is worth to apply and promote.