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为探讨内皮衍生因子 (NO/ET)与慢性胃溃疡形成的关系 ,采用大鼠醋酸性胃溃疡模型 ,分别以 L-精氨酸(L- Arg)和 NG-硝基 - L-精氨酸 (L- NNA)腹腔内注射 ,测定溃疡指数 ,以硝酸还原酶法和放射免疫技术 ,分别检测血清 NO和血浆 ET- 1含量。结果显示 :1L- Arg组溃疡指数显著低于模型组和 L- NNA组 (P<0 .0 1) ,L- NNA组溃疡指数显著高于模型组 (P<0 .0 1)。 2 L- Arg组血清 NO含量显著高于模型组及对照组 (P<0 .0 1) ,L- NNA组血清 NO含量显著低于以上 3组 (P<0 .0 1)。 3L- Arg组血浆 ET- 1水平显著低于模型组和对照组 (P<0 .0 1) ,L- NNA组血浆ET- 1水平显著高于以上各组 (P<0 .0 1)。提示内皮衍生因子的失衡可能是慢性胃溃疡形成的重要原因。
To investigate the relationship between endothelial derived factor (NO / ET) and the development of chronic gastric ulcer, rat model of gastric ulcer induced by acetic acid was established. L-arginine (L-Arg) and NG-nitro-L-arginine (L-NNA) were intraperitoneally injected to determine the ulcer index. Nitric acid reductase and radioimmunoassay were used to detect serum NO and plasma ET-1 levels respectively. The results showed that the ulcer index of 1L-Arg group was significantly lower than that of model group and L-NNA group (P <0.01), and the ulcer index of L-NNA group was significantly higher than that of model group (P <0.01). Serum NO levels in 2-Arg group were significantly higher than those in model group and control group (P <0.01). Serum NO level in L-NNA group was significantly lower than that in the above 3 groups (P <0.01). The level of plasma ET-1 in 3L-Arg group was significantly lower than that in model group and control group (P <0.01), while the level of ET-1 in LNA group was significantly higher than that of the above groups (P <0.01). Suggesting that the imbalance of endothelial-derived factor may be an important cause of chronic gastric ulcer formation.