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目的观察排铅聚糖对醋酸铅染毒小鼠的驱铅作用及对体内必需元素的影响。方法以昆明种小鼠为实验对象,按体质量随机分为阴性对照组,醋酸铅模型组,排铅聚糖高(1 600 mg/kg)、中(800 mg/kg)、低(400 mg/kg)剂量组,除阴性对照组外均腹腔注射醋酸铅溶液染毒造成中毒模型,后各剂量组用不同剂量排铅聚糖灌胃,阴性对照组及醋酸铅模型组用生理盐水灌胃。实验结束后取小鼠的血液、肝、肾、骨、脑组织进行消解,采用原子吸收分光光度法检测血液及各组织中铅及钙、镁、铁、锌、铜、锰的含量。结果血液及各组织脏器中铅的含量,排铅聚糖各剂量组均低于醋酸铅模型组(P<0.05)。排铅聚糖高剂量组血液、骨中镁的含量低于阴性对照组(P<0.05),肝铁含量中、高剂量组高于醋酸铅模型组(P<0.05),而与阴性对照组比较差异无统计学意义(P>0.05),排铅聚糖各剂量组中钙、铜、锌、锰与阴性对照组相比,在肝、肾、骨、脑中含量差异大部分无统计学意义(P>0.05)。结论排铅聚糖能有效地排出血液、肝、肾、骨、脑中的铅,但没有明显的剂量-效应关系。用排铅聚糖排出小鼠体内的铅时,除高剂量对血镁、骨镁有促排作用外,对机体内其他必需元素基本无损失,同时中、高剂量对肝内的铁有保护作用。
Objective To observe the lead excretion of lead excretion polysaccharides on mice induced by lead acetate and its effect on essential elements in the body. Methods Kunming mice were randomly divided into three groups according to the body weight: negative control group, lead acetate model group, high levan (1600 mg / kg), medium (800 mg / kg) and low (400 mg / kg) dose group, except the negative control group were injected intraperitoneal injection of lead acetate poisoning model, after each dose group with different doses of lead chitosan gavage, negative control group and lead acetate model group with normal saline gavage. After the experiment, the blood, liver, kidney, bone and brain tissue of mice were digested, and the content of lead, calcium, magnesium, iron, zinc, copper and manganese in blood and tissues were determined by atomic absorption spectrophotometry. Results The levels of lead and the contents of lead and levan in each group were lower than those in lead acetate group (P <0.05). The content of magnesium in blood and bone in the high-dose lead-releasing polysaccharide group was lower than that in the negative control group (P <0.05), middle and high-dose liver iron content group was higher than that in the lead acetate group (P <0.05) There was no significant difference between the two groups (P> 0.05). There was no significant difference in the contents of calcium, copper, zinc and manganese in liver, kidney, bone and brain among the groups (P> 0.05). Conclusion Reheparin can effectively excrete lead from blood, liver, kidney, bone and brain without obvious dose-effect relationship. With excretion of lead excretion of lead in mice, in addition to high doses of blood magnesium, bone magnesium has a role in promoting the discharge, the other essential elements of the body without any loss, while medium and high doses of iron on the liver to protect effect.