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经HPLC和氨基酸分析表明，采用单一溶媒分离环孢菌素D比多溶媒梯度洗脱柱层析收率更高，同时具有步骤简单、操作安全的优点。
The HPLC and amino acid analysis showed that cyclosporin D was separated with multi-solvent gradient elution column chromatography with a single solvent, and had the advantages of simple steps and safe operation.