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通过H2O2诱导人脐静脉内皮细胞(ECV-304)损伤,建立细胞氧化损伤模型。研究丝胶抗氧化肽段SP2、SP3低、中、高质量浓度(50,100,200μg/mL)对细胞免受损伤的保护作用。研究结果表明:SP2和SP3(100,200μg/mL)处理组显著提高了细胞中总抗氧化能力(P<0.05),SOD,CAT和GSH-Px酶水平(P<0.05)以及细胞的NO水平(P<0.05);显著降低了细胞中MDA水平(P<0.05)。SP2、SP3肽段对H2O2诱导人脐静脉内皮细胞(ECV-304)的损伤有很好的保护作用,存在剂量依赖关系。本研究为开发丝胶抗氧化肽功能产品提供了理论依据,为合理开发和利用丝胶提供了新的途径。
H2O2-induced injury of human umbilical vein endothelial cells (ECV-304), the establishment of cell oxidative damage model. To study the protective effect of sericin anti-oxidant peptides SP2 and SP3 on cell injury from low, medium and high concentration (50,100,200μg / mL). The results showed that the total antioxidant capacity (P <0.05), the activities of SOD, CAT and GSH-Px (P <0.05) and the levels of NO in the cells of SP2 and SP3 (100 and 200μg / mL) P <0.05), and significantly decreased the level of MDA in cells (P <0.05). SP2, SP3 peptide has a good protective effect on H2O2-induced injury of human umbilical vein endothelial cells (ECV-304) in a dose-dependent manner. This study provides a theoretical basis for the development of sericin antioxidant peptide functional products, which provides a new way for the rational development and utilization of sericin.