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目的了解十堰市从事禽类经营的职业人群与经营场所中禽流感病毒感染状况,为人禽流感防控提供依据。方法采集该市从事家禽规模养殖、销售和屠宰的职业人群血清118份,通过红细胞凝集抑制实验(HI)进行H5N1抗体检测;采集家禽规模养殖场和农贸活禽市场外环境标本171份,以real-time RT-PCR法检测禽流感病毒核酸Flu A、H5、H7、H9。结果职业人群H5N1(A/Chiken/Hong Kong/Ap156/2008)抗体阳性率为15.25%;经营场所外环境中Flu A、H5、H9在不同季节检出率依次为5.00%~57.50%、0.00%~25.00%和2.50%~50.00%,未检出H7;规模养殖场标本Flu A核酸均为阴性,农贸活禽市场标本Flu A、H5、H7、H9及H5与H9混合阳性核酸检出率依次为:46.85%、16.22%、0.00%、43.24%和14.41%。结论十堰市禽类经营职业人群存在一定的H5N1隐性感染,经营场所存在H5、H9亚型禽流感病毒的污染,应加强职业人群的禽流感监测与防护,落实农贸活禽市场的“三个一措施”。
Objective To understand the prevalence of avian influenza virus in occupational groups and establishments engaged in the operation of poultry in Shiyan City, and provide basis for the prevention and control of human bird flu. Methods A total of 118 serum samples were collected from the occupational population engaged in scale farming, marketing and slaughtering in the city. H5N1 antibody was detected by hemagglutination inhibition (HI) assay. 171 environmental samples were collected from poultry scale farms and farmer live poultry market, Fluorescence Fluids A, H5, H7 and H9 were detected by -time RT-PCR. Results The prevalence of H5N1 (A / Chiken / Hong Kong / Ap156 / 2008) antibody was 15.25% in occupational groups. The detection rates of Flu A, H5 and H9 in different occupations were 5.00% -57.50%, 0.00% ~ 25.00% and 2.50% ~ 50.00%, respectively. H7 was not detected. Flu A nucleic acid was negative in the scale farm samples. The positive rate of mixed samples of Flu A, H5, H7, H9, H5 and H9 As follows: 46.85%, 16.22%, 0.00%, 43.24% and 14.41%. Conclusion There is a certain H5N1 recessive infection in the occupational population of poultry in Shiyan and the presence of H5 and H9 subtype avian influenza virus in the business sites. Monitoring and prevention of avian influenza should be strengthened in the occupational population. Three “ A measure ”.