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目的 :研究巴曲抗栓酶 [batroxobin(DF 52 1) ]对沙土鼠脑缺血再灌注期间纹状体多巴胺(dopamine ,DA)和海马ATP含量变化的影响。方法 :阻断沙土鼠双侧颈总动脉制备前脑缺血再灌注模型 ,沙土鼠 4 0只分成 5组 ,假手术组、脑缺血组、对照组、巴曲抗栓酶Ⅰ组 (8BU·kg- 1)和巴曲抗栓酶Ⅱ组 (16BU·kg- 1) ,每组 8只。沙土鼠于脑缺血10min或再灌注 6 0min时处死 ,分别测定各组海马ATP ,ADP ,AMP含量和纹状体多巴胺的含量。结果 :脑缺血组海马ATP含量和纹状体DA含量均明显低于假手术组。对照组 (0 .9%氯化钠注射液 )再灌注 6 0min时纹状体DA与海马ATP含量明显高于脑缺血组 ,但明显低于巴曲抗栓酶Ⅰ ,Ⅱ组。结论 :巴曲抗栓酶可促进脑缺血再灌注期间海马ATP含量的恢复和减少纹状体多巴胺的释放
Objective: To study the effect of batroxobin (DF 52 1) on ATP content in dopamine (DA) and hippocampus during gerbil brain ischemia / reperfusion. Methods: The model of forebrain ischemia-reperfusion was established by blocking bilateral common carotid arteries of gerbils. 40 gerbils were divided into 5 groups: sham operation group, cerebral ischemia group, control group, · Kg -1) and batroxobin Ⅱ group (16BU · kg -1), with 8 rats in each group. Gerbils were sacrificed at 10 min after ischemia or 60 min after reperfusion. The levels of ATP, ADP and AMP in the hippocampus and dopamine in the striatum were determined. Results: The contents of ATP and DA in hippocampus in cerebral ischemia group were significantly lower than those in sham group. The ATP content of DA and hippocampus in striatum of the control group (0.9% sodium chloride injection) at 60 min reperfusion was significantly higher than that of the cerebral ischemia group, but significantly lower than that of the batroxobin Ⅰ and group Ⅱ. CONCLUSION: Batroxobin can promote the recovery of ATP content in the hippocampus and decrease the release of striatum dopamine during cerebral ischemia-reperfusion