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[目的]研究异槲皮苷对自发性糖尿病小鼠肝脏脂肪酸代谢相关基因表达的调控作用。[方法]8周龄雄性db/db小鼠随机分为模型组、异槲皮苷组,雄性C57BL/6J小鼠为正常对照组,药物干预4周后,检测小鼠体质量、空腹血糖(FBG)、总胆固醇(CHO)、甘油三酯(TG)、游离脂肪酸(FFA),肝质量和肝质量/体质量;肝组织苏木精-伊红(HE)染色观察肝细胞脂质蓄积;逆转录聚合酶链式反应(RT-PCR)基因表达。[结果]与模型组比较,异槲皮苷组小鼠体质量、FBG、CHO、TG、FFA、肝质量、肝质量/体质量显著降低(P<0.05或P<0.01);苏木精-伊红(HE)染色肝细胞脂肪变性减轻,肝脏SREBP1c、FAS m RNA表达降低(P<0.05;P<0.01),PGC1α、PPARαm RNA表达显著升高(P<0.05)。[结论]异槲皮苷可以改善db/db小鼠肝脏脂肪酸代谢紊乱,可能是通过下调肝脏SREBP1c、FASm RNA表达,上调肝脏PGC1α、PPARα表达实现的。
[Objective] To study the regulatory effect of isoquercitrin on hepatic fatty acid metabolism-related gene expression in spontaneously diabetic mice. [Methods] Eight weeks old male db / db mice were randomly divided into model group, isoquercitrin group and male C57BL / 6J mice as normal control group. After intervention for 4 weeks, the body weight, fasting blood glucose (FBG), total cholesterol (CHO), triglyceride (TG), free fatty acid (FFA), liver mass and liver mass / body weight were measured. Liver tissue was stained with hematoxylin- Reverse transcription polymerase chain reaction (RT-PCR) gene expression. [Results] The body weight, FBG, CHO, TG, FFA, liver mass and liver mass / body weight were significantly decreased in the isoquercitrin group compared with the model group (P <0.05 or P <0.01) Eosin (HE) staining reduced the fatty degeneration of hepatic cells, decreased the expression of hepatic SREBP1c and FASmRNA (P <0.05; P <0.01), and increased the expressions of PGC1α and PPARαmRNA (P <0.05). [Conclusion] Isoquercitrin can improve hepatic fatty acid metabolism disorder in db / db mice, which may be through down-regulating the expression of SREBP1c and FASmRNA in liver and up-regulating the expression of PGC1α and PPARα in liver.