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目的观察一氧化氮(NO)及其清除剂对舌下神经运动核(HMN)活动的影响并初步探讨其机制。方法麻醉雄性成年Wistar大鼠,微透析探针先透析人工脑脊液(ACSF)到HMN作为对照,然后再分别持续给予二乙胺一氧化氮水合钠盐(DEA,NO供体)和2-(4-羧基苯基)-4,4,5,5,-四甲基-1,3-二氧咪唑啉钾盐(carboxy-PTIO,NO清除剂)到HMN,观测大鼠颏舌肌(GG)、膈肌电活动及血压和呼吸频率的变化。结果给予DEA后,大鼠的呼吸相关GG活动明显增加,而给予carboxy-PTIO后则明显降低,与ACSF时的值相比差异均有统计学意义(P<0.05)。不管是给予DEA还是carboxy-PTIO,在30~120 min时大鼠的紧张性GG活动、膈肌活动、血压及呼吸频率与ACSF时的值相比均无统计学差异(P>0.05)。结论 NO作为一种神经递质对HMN产生兴奋,从而对维持生理状态下上气道的开放起重要作用。
Objective To observe the effect of nitric oxide (NO) and its scavenger on the activity of hypoglossal motor nucleus (HMN) and to explore its mechanism. Methods Male adult Wistar rats were anesthetized. Microdialysis dialysis was performed on dialyzed artificial cerebrospinal fluid (ACSF) to HMN as control. Then, diethylamine monoamine oxidase (DEA, NO donor) and 2- (4 - carboxylate) -4,4,5,5 - tetramethyl-1,3-dioxoimidazoline potassium salt (carboxy-PTIO, NO scavenger) to HMN, observe the genioglossus muscle (GG) , Diaphragm electrical activity and changes in blood pressure and respiratory rate. Results After administration of DEA, respiration-related GG activity was significantly increased in rats, but decreased significantly after carboxy-PTIO administration. The difference was statistically significant (P <0.05). No significant difference was found in the activity of GG, diaphragmatic muscle activity, blood pressure and respiratory rate between the two groups at 30 ~ 120 min (P> 0.05), no matter whether DEA or carboxy-PTIO was administered. CONCLUSIONS: NO as a neurotransmitter excites HMN and plays an important role in maintaining the upper airway opening in the physiological state.