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目的:探讨14-3-3β蛋白在慢性宫颈炎、宫颈上皮内瘤样病变(CIN)、宫颈癌(CC)中的表达情况。方法:采用逆转录聚合酶链反应(RT-PCR)方法检测14-3-3β蛋白mRNA在慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ、宫颈癌的表达情况,采用图像分析软件处理数据,用系统统计软件SPSS 11.5进行统计分析。结果:14-3-3β蛋白mRNA在慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ、宫颈癌的表达量分别为0.61±0.07、0.69±0.03、1.11±0.13、1.29±0.08、1.45±0.02;CC组、CINⅢ组、CINⅡ组与慢性宫颈炎组相比,表达明显上升,有显著性差异(P<0.01);CC组与CIN各组相比,表达上升,有显著性差异(P<0.01);CIN组间随着病变程度的增加,表达呈上调趋势,有差异(P<0.05);CINⅠ组与慢性宫颈炎组相比,表达无差异性(P>0.05)。结论:14-3-3βmRNA在CINⅡ、CINⅢ和宫颈癌中表达明显上调,有利于临床指导宫颈癌的早期诊断和治疗。
Objective: To investigate the expression of 14-3-3β protein in chronic cervicitis, cervical intraepithelial neoplasia (CIN) and cervical cancer (CC). Methods: The expression of 14-3-3β mRNA in chronic cervicitis, CINⅠ, CINⅡ, CINⅢ and cervical cancer was detected by reverse transcription polymerase chain reaction (RT-PCR). The data were processed by image analysis software, SPSS 11.5 software for statistical analysis. Results: The expression levels of 14-3-3β mRNA in chronic cervicitis, CINⅠ, CINⅡ, CINⅢ and cervical cancer were 0.61 ± 0.07,0.69 ± 0.03,1.11 ± 0.13,1.29 ± 0.08 and 1.45 ± 0.02 respectively. In CC group, The expression of CINⅢand CINⅡwere significantly higher than that of chronic cervicitis group (P <0.01). The expression of CINⅢand CINⅡwere significantly higher than that of CINⅢand CINⅡgroup (P <0.01) There was a significant difference between the two groups (P <0.05). There was no significant difference between the CINⅠgroup and the chronic cervicitis group (P> 0.05). Conclusion: The expression of 14-3-3β mRNA in CINⅡ, CINⅢ and cervical cancer is significantly up-regulated, which is helpful for the early diagnosis and treatment of cervical cancer.