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本实验以~3H-TdR(~3H-胸腺嘧啶核苷)掺入法研究了相同碳链长度的硒化不饱和脂酸及相应未硒化脂酸对7402人肝癌细胞株DNA合成的抑制作用。实验证明,终浓度为150ug/ml的受试物对人肝癌细胞株DNA合成的抑制作用以硒化亚麻酸量强,与硒化亚油酸、硒化油酸和油酸相比,差异非常显著(P<0.01),与亚油酸相比,差异显著(P<0.05),但与亚麻酸相比,差异未达统计学的显著程度(P>0.05),硒化亚油酸与亚油酸之间无差异(P>0.05),而硒化油酸与油酸则差异非常显著(P<0.01)。表明:含2-3个双键的脂酸,硒化与否,抑制癌细胞DNA合成强度差异不大,但对仅含一个双键者,硒化可显著增强对DNA合成的抑制作用。各种受试物对人肝癌细胞株的杀伤作用也以硒化亚麻酸最强。
In this study, ~3H-TdR (~3H-thymidine) incorporation method was used to study the inhibitory effect of selenated unsaturated fatty acids and corresponding unselenized fatty acids with the same carbon chain length on DNA synthesis of 7402 human hepatoma cell lines. . Experiments show that the final concentration of 150ug/ml of the test substance on human hepatoma cell line DNA synthesis inhibitory effect of selenium linolenic acid, and selenized linoleic acid, selenized oleic acid and oleic acid, the difference is very Significant (P<0.01), compared with linoleic acid, the difference was significant (P<0.05), but the difference was not statistically significant (P>0.05) compared to linolenic acid, selenized linoleic acid and There was no difference between oleic acid (P>0.05), but the difference between selenated oleic acid and oleic acid was very significant (P<0.01). The results showed that the selenization of 2-3 double-stranded fatty acids did not significantly inhibit the DNA synthesis of cancer cells, but selenation significantly enhanced the inhibition of DNA synthesis in those containing only one double bond. The killing effect of various test substances on human hepatoma cell lines was also the strongest with selenated linolenic acid.