论文部分内容阅读
用我国自己克隆的恶性疟原虫ＤＮＡ，分别用同位素和生物素标记后用作探针，检测了采自海南、云南和山东省的血样。镜检的符合率：恶性疟９８．３％，间日疟８８．９％，混合感染血１００％，正常人血９９．９％。探针检测敏感度为８４个原虫／μ１血；同位素标记比生物素标记敏感１００倍；可用放置２年的血样，不必提取ＤＮＡ直接点在硝酸纤维素膜上可进行检测，效率比镜检提高１０倍以上。研究表明，ＤＮＡ探针技术可做为疟疾监测的重要手段。
With our own cloned Plasmodium falciparum DNA, were labeled with isotopes and biotin used as a probe, the detection of blood samples collected from Hainan, Yunnan and Shandong Province. Microscopic examination of the coincidence rate: 98.3% of falciparum malaria, vivax malaria 88.9%, mixed infection of blood 100%, normal blood 99.9%. The detection sensitivity of the probe was 84 protozoa / μl blood; the isotope labeling was 100 times more sensitive than the biotin labeling; the blood sample that could be placed for 2 years could be detected directly on the nitrocellulose membrane without extracting the DNA, and the efficiency was higher than the microscopic examination 10 times more. Studies have shown that DNA probe technology can be an important tool for malaria surveillance.