大麦黄花叶病毒的机械接种一般发病率较低,为提高其发病率,我们进行了缓冲液种类、用量,接种源种类,接种时苗龄,按种后苗培育时的温度、光照强度和时数等因素的研究。接种后麦苗在18℃恒温条件下,最早在第7天开始发病,第15—20天病株增加较多,到第30天病株稳定不再增加。(一)缓冲液种类和用量。在强光下,以0.1M pH7.0 PBS(Na_2HPO_4+KH_2PO_4)加0.01M DIECA The general incidence of mechanical inoculation of barley yellow mosaic virus is low, in order to improve its incidence, we carried out the buffer type, dosage, species of inoculation, seedling age when inoculated, according to seedling temperature after incubation, light intensity and time Number of factors such as research. After inoculation, the wheat seedlings started to develop as early as the 7th day under the constant temperature of 18 ° C, and the number of the plants increased more from the 15th day to the 20th day. Stable no longer increased on the 30th day. (A) the type and amount of buffer. In bright light, 0.1 M pH 7.0 PBS (Na 2 HPO 4 + KH 2 PO 4) plus 0.01 M DIECA