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在生物学、医学研究中,十分注意H~+、Na~+、K~+、Ca~(2+)等离子活度的测量,目前普遍采用玻璃电极测量H~+和Na~+活度,而K~+、Ca~(2+)是用液体膜电极测定,这两种电极都有输出阻抗太高、响应慢、易碎、体积大的缺点。 1978年,Moss等人首先报导了以缬氨霉素为活性物质制作的PVC膜钾离子敏感场效应晶体管。但缬氨霉素难合成,价钱昂贵。我们采用二苯并-18-王冠-6为活性物质,试验成功PVC膜钾离子敏感场效应晶体管,其线性响应范围10~0～10~(-5)M,灵敏度为43mV/pK,选择比K_(NaK)>100;超过MOSS等人制作的钾ISFET的响应范围(10~(-1)～10~(-3)M)。钾离子敏感场效应晶体管具有体积小、响应快、输出阻抗低等优点。
In biology and medical research, great attention has been paid to the measurement of plasma activity of H ~ +, Na ~ +, K ~ + and Ca ~ (2+). At present, the glass electrode is used to measure H ~ + and Na ~ + activity, The K ~ +, Ca ~ (2+) is measured by the liquid membrane electrode. Both electrodes have the disadvantages of too high output impedance, slow response, fragile and large volume. In 1978, Moss et al. First reported on PVC membrane potassium ion sensitive field effect transistors made of valinomycin. But valinomycin difficult synthesis, expensive. We use dibenzo-18-crown-6 as the active substance, the successful test of PVC membrane potassium ion-sensitive field-effect transistor, the linear response range of 10 ~ 0 ~ 10 ~ (-5) M, the sensitivity of 43mV / pK, the selectivity K_ (NaK)> 100; exceeding the response range (10 ~ (-1) ~ 10 ~ (-3) M) of potassium ISFET made by MOSS et al. Potassium ion-sensitive field effect transistor has the advantages of small size, fast response, low output impedance.