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目的比较维吾尔族不同龋敏感儿童变链菌临床分离株生物膜状态和浮游状态致龋能力的差异。方法根据析因实验设计分组,选取课题组前期分离鉴定的维吾尔族儿童变链菌临床株27株,其中17株高龋菌株[龋失补牙数(dmft)≥5],10株无龋菌株[龋失补牙数(dmft)=0]。分别在24孔板静止培养以及在试管内摇动培养。采用蒽酮法测定高龋组与无龋组分别在生物膜状态和浮游状态下合成胞外多糖的量。使用SPSS 17.0软件包对实验结果进行析因分析。结果高龋组生物膜状态下合成水溶性与非水溶性葡聚糖量分别为(0.3011±0.0398)mg/mL、(0.3711±0.0372)mg/mL,高于浮游状态的(0.2300±0.0438)mg/mL、(0.2356±0.0568)mg/mL,无龋组生物膜状态下合成水溶性与非水溶性葡聚糖分别为(0.2067±0.0264)mg/mL、(0.3489±0.0537)mg/mL,高于浮游状态的(0.1489±0.0325)mg/mL、(0.1578±0.0227)mg/mL,差异有统计学意义(P<0.05)。并且高龋组合成胞外多糖量均高于无龋组,差异有统计学意义(P<0.05)。结论维吾尔族高龋儿童变链菌临床分离株的高致龋力可能与其携带有合成胞外多糖能力较强的菌株有关。
Objective To compare the differences of cariogenic ability between Streptococcus mutans isolates and biofilm status in caries-sensitive children with Uygur caries. Methods According to the design of factorial experiment, 27 clinical strains of Streptococcus mutans of Uygur children, 17 strains of cariogenic strains [dental caries missing teeth (dmft) ≥5] and 10 cariogenic strains [ Dental caries (dmft = 0]. Respectively in 24-well plate static culture and shake culture in vitro. The anthrone method was used to determine the amount of extracellular polysaccharides synthesized in the biofilm and pelagic states of the caries-free and caries-free groups, respectively. The experimental results were analyzed using SPSS 17.0 software package. Results The results showed that the amount of water-soluble and water-insoluble glucosans in the biofilm group was (0.3011 ± 0.0398) mg / mL and (0.3711 ± 0.0372) mg / mL, respectively, higher than that in the plankton (0.2300 ± 0.0438) mg / mL, (0.2356 ± 0.0568) mg / mL. The water-soluble and non-water-soluble dextran in the caries-free biofilm were 0.2067 ± 0.0264 mg / mL and 0.3489 ± 0.0537 mg / (0.1489 ± 0.0325) mg / mL, (0.1578 ± 0.0227) mg / mL in the floating state, the difference was statistically significant (P <0.05). The amount of exopolysaccharide combined with high caries was higher than that without caries, the difference was statistically significant (P <0.05). Conclusion The high cariogenic potential of Streptococcus mutans isolates from Uigur caries children may be related to their strong ability to carry exopolysaccharide.