Wyss 等人和 Berger 先后报道过叠氮化钠(NaN_3)提高了金黄葡萄球菌的抗青霉素和链霉素突变体的频率。Sqence 首次报道了 NaN_3在大麦上诱发6%的叶绿素缺乏突变频率(以M_1穗为单位,下同)。Sideris 等把处理溶液的 pH 值降到 NaN_3的等电点(pH4.8)后,使叶绿素突变频率提高到20%。Nilan 等和 Kleiahofs 等报道了利用20℃下预浸种子4小 Wyss et al. And Berger have reported that sodium azide (NaN_3) has been reported to increase the frequency of S. aureus penicillin and streptomycin mutants. For the first time, Sqence reported the frequency of NaN_3-induced chlorophyll deficiency mutations in barley at 6% (in M_1 spike, the same applies below). Sideris et al reduced the pH of the treatment solution to the isoelectric point of NaN_3 (pH 4.8) and increased the frequency of chlorophyll mutations to 20%. Nilan et al. And Kleiahofs et al. Reported using pre-impregnated seeds at 20 ° C for 4 hours